Research Protocol

H. pylori — The One Gut Bug You Should Actually Treat With Antibiotics

A research protocol for Helicobacter pylori — where the natural arsenal genuinely helps, and where using it instead of eradication therapy is a mistake with a cancer risk attached.

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HomeResearch Protocols › H. pylori
Research & educational use only. The information below summarizes published research and mechanisms. It is not medical advice or a recommendation for human use. Application and protocols are provided to Skool members.

Who this is for

Burning upper abdominal pain, especially on an empty stomach. Early fullness, nausea, reflux that never quite responds. A family history of gastric cancer. Or you tested positive and want to understand what you are actually dealing with.

Not for you if: Vomiting blood, black tarry stools, difficulty swallowing, unintentional weight loss, or anaemia. Those are urgent endoscopy signs, today, not protocol signs.

The thing this whole protocol turns on

This is the one where you take the antibiotics.

Almost everything else on this site is a case for doing something other than a prescription. This is the exception. H. pylori is a World Health Organization class I carcinogen — the strongest identified risk factor for gastric cancer, and eradicating it measurably reduces that risk. It also causes most peptic ulcers. Treating a confirmed infection with herbs instead of eradication therapy is not a lifestyle choice; it is leaving a carcinogen in place. Everything below is for supporting eradication, improving tolerance of it, or holding the line if you have failed therapy twice — not for replacing it.

The current ACG guideline sets out which regimens actually work now that clarithromycin resistance has climbed, and confirming eradication afterwards is part of the guideline, not optional. (PMID 39626064)

The natural arsenal belongs in arm three. Used as arm two it is the one genuinely dangerous mistake on this whole site.

Why eradication fails, and it fails often

What your labs are telling you

What to look atWhy it matters here
Urea breath test or stool antigenThe two non-invasive tests worth having. Both need you off PPIs for two weeks and off antibiotics for four, or they read falsely negative.
Serology (blood antibody)The weakest option — it stays positive after successful treatment, so it cannot confirm eradication or distinguish current from past infection.
CBC + ferritinH. pylori is a genuine and frequently missed cause of iron deficiency that will not respond to iron alone.
Vitamin B12Chronic atrophic gastritis from long-standing infection impairs B12 absorption.
CMPBaseline before a two-week multi-drug regimen.

Test before you treat

You cannot tell which phenotype you have, or whether you are actually malabsorbing, without numbers. This is the Gut Health & Absorption panel — 11 markers, loaded into the cart in one click with Cam’s code already applied.

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Load the Gut Health & Absorption panel →

Drawn at Quest. Results in days, not weeks. Paste them into the Bloodwork Vault when they land.

The one with real human eradication data

Sulforaphane-rich broccoli sprouts reduced H. pylori colonisation and attenuated gastritis in both mice and humans — an unusually clean piece of translational work for a food. An earlier preliminary trial of oral broccoli sprouts pointed the same way. This does not eradicate the organism on its own, and the papers do not claim it does.

PMID 19349290 PMID 15387326

Sulforaphane (Crucera-SGS)Clinical

A stabilized broccoli-seed precursor to sulforaphane, the most potent natural activator of Nrf2 — the master switch for the body's own antioxidant and detox enzymes.

The trials used fresh broccoli sprouts, roughly 70g a day for eight weeks — a food, not a capsule, and cheap. The supplement above is the standardised version for people who will not eat sprouts daily for two months.

The adjuncts with meta-analysis behind them

Lactoferrin supplementation alongside standard eradication therapy improved eradication rates and reduced adverse events in a meta-analysis — note alongside, not instead of. Saccharomyces boulardii is the best-supported probiotic for making the regimen tolerable, which matters because the most common reason therapy fails is that people stop taking it.

PMID 19298339 PMID 37111484

LactoferrinClinical

An iron-binding immune protein that supports gut and systemic immunity, regulates iron, and has antimicrobial/antiviral activity.

Saccharomyces boulardiiClinical

A beneficial probiotic YEAST (not a bacterium) that is uniquely resistant to antibiotics — making it ideal support during and after antibiotic courses.

L. reuteriCorrelative

A specific probiotic species with genuinely strain-specific effects.

Mastic gum — and the study that disagrees

This is the honest one. A randomised pilot found mastic gum had an effect on H. pylori; a separate in-vivo study found no effect on bacterial load at all. Two studies, opposite conclusions, neither large. Anyone selling you mastic gum as an H. pylori cure is quoting one and not the other.

PMID 19879118 PMID 12888582

Mastic GumCorrelative

Tree resin from the Greek island of Chios with a small but real evidence base in upper-GI symptoms.

DGL LicoriceCorrelative

Licorice with the blood-pressure-raising glycyrrhizin removed — soothes the stomach and supports the mucosal lining for reflux and ulcers.

Zinc CarnosineClinical

A zinc-and-carnosine complex that adheres to and heals the stomach and gut lining — excellent for ulcers, gastritis and leaky gut.

Mucosal support and repair

Once the organism is gone, the stomach lining has usually been inflamed for years. This arm is about the repair phase and about making the two weeks of therapy survivable.

PMID 16777920

BerberineCorrelative

A plant alkaloid that activates AMPK — the same energy-sensing pathway as exercise and metformin — with powerful effects on glucose and lipid metabolism.

Oregano OilTheoretical

A potent antimicrobial essential oil (carvacrol/thymol) used short-term for gut-flora balance and immune defense.

MonolaurinTheoretical

A monoglyceride of lauric acid, popular in antiviral and antimicrobial protocols.

PropolisTheoretical

The resin bees use to seal the hive — antimicrobial by evolutionary necessity, and with a reasonable amount of human data for topical and oral use.

Manuka HoneyTheoretical

Honey with unusually high methylglyoxal content.

Black Seed OilCorrelative

A traditional 'cure for everything but death' seed oil (thymoquinone) with real evidence for immune, metabolic and anti-inflammatory support.

Green Tea (EGCG)Theoretical

Concentrated green-tea catechins (EGCG) in an absorption-enhanced phytosome — a potent antioxidant with metabolic interest.

CurcuminCorrelative

The active polyphenol of turmeric, in Thorne's phytosome (Meriva) delivery that dramatically improves its otherwise poor absorption.

Vitamin CCorrelative

Water-soluble antioxidant and essential cofactor for collagen synthesis, immune cells and neurotransmitter production.

L-GlutamineCorrelative

The most abundant amino acid in the body and a primary fuel for gut and immune cells.

What to stop wasting money on

The 8-week protocol is inside Skool

Everything above is yours free — what it is, why it recurs, what to test, and every compound by name. What is inside is what to actually do with them.

Join Skool — $10/mo →

Retest, and what “working” looks like

At 4+ weeks after the last antibiotic, off PPIs for 2 weeks, re-run the panel above.

Working:

Not working: A positive confirmation test. That is a resistance problem and needs a different regimen, not more of the same one.

See a doctor, not a protocol, if: Vomiting blood or coffee-ground material. Black tarry stools. Difficulty or pain swallowing. Unintentional weight loss. Anaemia. New dyspepsia over 55. A first-degree relative with gastric cancer. Any of those is an endoscopy conversation now.

Where to go next

The H. pylori goal pathways map the same territory by mechanism rather than by condition, and the gut blueprint is the broader stack this protocol sits inside.

Not getting anywhere on your own?

This page is a template, and a template cannot see your history, your labs or the three things you already tried. Tell Cam where you are now and he will build the version of this that fits you.

Questions people actually ask

Can you treat H. pylori naturally without antibiotics?

You should not try to, if the infection is confirmed. H. pylori is classified as a group I carcinogen and is the strongest known risk factor for gastric cancer, and eradication reduces that risk. Some natural agents have real supporting data — sulforaphane from broccoli sprouts reduced colonisation in humans, and lactoferrin improved eradication rates in a meta-analysis — but both were studied as support for standard therapy, not as a replacement for it.

Do broccoli sprouts kill H. pylori?

They reduce it. Dietary sulforaphane-rich broccoli sprouts lowered colonisation and reduced gastritis markers in infected humans as well as in mice. What the research shows is suppression and reduced inflammation, not eradication, and the effect faded after supplementation stopped. It is a genuine adjunct with human data behind it, which makes it unusual in this category.

Does mastic gum work for H. pylori?

The evidence contradicts itself. A randomised pilot study found an effect; a separate in-vivo study found no effect on bacterial load at all. Both were small. Anyone presenting mastic gum as an established H. pylori treatment is citing one study and not the other. It has better evidence for general upper-GI symptom relief than for eradication.

Why does H. pylori treatment fail?

Mostly antibiotic resistance — clarithromycin resistance in particular has risen enough that older triple therapy now fails a substantial share of the time, which is why current guidelines moved away from it. The other big cause is not completing the course, because the regimens are genuinely unpleasant. Failing to confirm eradication afterwards means people also believe they have been cured when they have not.

When should you retest after H. pylori treatment?

At least four weeks after finishing antibiotics, and after at least two weeks off any proton pump inhibitor, using a urea breath test or stool antigen test. Blood antibody testing cannot be used to confirm eradication because antibodies persist long after the organism has gone.

Sources

Every reference below was resolved against the NCBI PubMed API on 23 August 2026 — title, journal and year read back from NCBI rather than from memory.

  1. PMID 39626064 — Chey WD. ACG Clinical Guideline: Treatment of Helicobacter pylori Infection. Am J Gastroenterol, 2024.
  2. PMID 19349290 — Yanaka A. Dietary sulforaphane-rich broccoli sprouts reduce colonization and attenuate gastritis in Helicobacter pylori-infected mice and humans. Cancer Prev Res, 2009.
  3. PMID 15387326 — Galan MV. Oral broccoli sprouts for the treatment of Helicobacter pylori infection: a preliminary report. Dig Dis Sci, 2004.
  4. PMID 19298339 — Zou J. Meta-analysis: the effect of supplementation with lactoferrin on eradication rates and adverse events during Helicobacter pylori eradication therapy. Helicobacter, 2009.
  5. PMID 37111484 — Imoto I. Antimicrobial effects of lactoferrin against Helicobacter pylori infection. Pathogens, 2023.
  6. PMID 19879118 — Dabos KJ. The effect of mastic gum on Helicobacter pylori: a randomized pilot study. Phytomedicine, 2010.
  7. PMID 12888582 — Bebb JR. Mastic gum has no effect on Helicobacter pylori load in vivo. J Antimicrob Chemother, 2003.
  8. PMID 16777920 — Mahmood A. Zinc carnosine, a health food supplement that stabilises small bowel integrity and stimulates gut repair processes. Gut, 2007.